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Determination the Content of Baicalin in Huaihuang Pills by HPLC
Author(s): 
Pages: 25-26
Year: Issue:  5
Journal: WORLD HEALTH DIGEST·MEDICAL MONTHLY

Keyword:  高效液相色谱法槐黄丸黄芩苷;
Abstract: 目的建立高效液相色谱法测定槐黄丸中黄芩苷含量的方法.方法:色谱柱:Ultimate XB-C18柱(5μm,4.6×250mm);流速为1.0m1.mm-1;柱温:30℃;流动相:甲醇-水-磷酸(47∶53∶0.2);检测波长:280nm;进样量:10μl.结果:黄芩苷在10.25~164.00μg.ml-1范围内呈良好的线性关系(r=0.9999),平均回收率(n=6)为100.78%(RSD=I.60%).结论:该方法简便、准确,专属性强,可以作为槐黄丸的质量控制.
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