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zhong xian jing dian shi yan gan wu xian ren zhi hui pei yang ke xue si wei kai fang shi yan zhi kang yi yan zuo de zuo duo chun gao chan jun zhu de ding xiang pei yu
Author(s): 
Pages: 310-311
Year: Issue:  5
Journal: WORLD HEALTH DIGEST·MEDICAL MONTHLY

Keyword:  开放实验经典实验自发突变诱变育种;
Abstract: 开放实验室是改革实验课教学,提高学生综合能力的主要途径之一,而经典实验是学生设计实验方案的优秀范例.本实验室开展了突变与育种这一微生物学经典实验,通过梯度平板法阶段筛选双歧杆菌的抗高浓度异烟肼自发突变株,在自发突变的基础上,通过紫外线和亚硝基甲基脲(NMU)处理对抗异烟肼的突变株进行诱变,再通过梯度培养法筛选到抗更高浓度异烟肼的突变体.从而重现了经典实验,使学生对变异的特征以及变异在生物生产上的应用有更深的了解,并让学生受到科学思维方法的熏陶和引导,培养了他们提出假设、设计实验的能力.
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