AcSDKP attenuates PDGF-induced cadiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway
Author(s): ZHANG Li-juan, MA Wen-dong, YANG Yan, WANG Rui-min, CHEN Ping, YAN Jing-bo, LI Qian, LI Dan-dan, YANG Fang
Pages: 444-448
Year: 2008 Issue: 5
Journal: CHINESE JOURNAL OF CARDIOLOGY
Keyword: Heart; Fibrosis; Extracellular signal-regulated MAP kinase; Fibroblasts; Collagen;
Abstract: Objective To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2(ERK1/2)pathway on this process.Methods Metabolic activity of fibroblasts was determined by CCK-8.Cell cycle was detected by flow cytometry.Expressions of type Ⅰand type Ⅲ collagen were measured by immunocytochemistry and Western blot.Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot. Result 10-9 mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2.while the protein levels of ERK1/2 were not significantly affected by AcSDKP.Conclusion AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
Pages: 444-448
Year: 2008 Issue: 5
Journal: CHINESE JOURNAL OF CARDIOLOGY
Keyword: Heart; Fibrosis; Extracellular signal-regulated MAP kinase; Fibroblasts; Collagen;
Abstract: Objective To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2(ERK1/2)pathway on this process.Methods Metabolic activity of fibroblasts was determined by CCK-8.Cell cycle was detected by flow cytometry.Expressions of type Ⅰand type Ⅲ collagen were measured by immunocytochemistry and Western blot.Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot. Result 10-9 mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2.while the protein levels of ERK1/2 were not significantly affected by AcSDKP.Conclusion AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
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