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Detection of streptomycin resistance in Mycobacterium tuberculosis clinical isolates by denaturing high-performance liquid chromatography and DNA sequencing
Pages: 1376-1379
Year: Issue:  20

Keyword:  Mycobacteriun tuberculosisDrug resistancebacterialChromatographyhigh pressure liquidMicrobial sensitivity test;
Abstract: Objective To determine the rpsL and rrs gene mutation in Mycobacterium tuberculosis (M. tuberculosis)and compare the consistency between the results of denaturing high-performance liquid chromatography(DHPLC)and those of DNA sequencing. Methods The values of streptomycin minimum inhibitory concentration(MIC)against 215 M. tuberculosis clinical isolates, 115 being streptomycinresistant and 100 being susceptible by a routine proportional method, were tested by DHPLC. DNA sequencing was conducted to detect the rpsL and rrs mutation. Results 98 of the 115 streptomycin-resistant isolates(85. 2%)harbored rpsL and/or rrs mutation, 76. 5%of which being rpsL mutation(88/115). There was no significant correlation between the MIC values and mutation types. No mutation was found in all the susceptible isolates. There was a complete consistency between the DHPLC results and those of DNA sequencing. Conclusion DHPLC can be regarded as a useful and powerful tool to detect the streptomycin resistance detection in M. tuberculosis.
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