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Effect of non-restrictive external stent on the expression of platelet-derived growth factor in vein grafts of rabbits
Author(s): 
Pages: 1418-1421
Year: Issue:  20
Journal: NATIONAL MEDICAL JOURNAL OF CHINA

Keyword:  StentsPlatelet-derived growth factorTransplantationautologous;
Abstract: Objective To investigate the potencial mechanism of non-restrictive external stent in preventing re-stenosis of vein grafts.Methods Thirty-six rabbits underwent reversed bypass grafting of autologous external iugular vein into common carotid artery and then were randomly divided into two equal groups,stenting group(Group S),with an external stent 6 mm in diameter(Dacron vascular prosthesis)surrounding the vein graft,and control group(Group NS),without stenting.The vein grafts were harvested 7.14.and 28 days after the operation respectively.Immunocytochemistry was used to detect the plateletderived growth factor B(PDGF-B)positive cells.The mRNA expression of PDGF-B was detected with RTPCR.Results The PDGF-B positive cell percentages in the intima of Group S7 and 14 days later were both significantly lower than those of Group NS(15.2%±3.6%vs 21.6%±4.6%.and 6.5%±2.6%vs12.5%±4.4%,both P<0.05).The PDGF-B positive cell percentages in media 7,14 and 28 days later of Group S were all significantly lower than those of Group NS(13.8%±4.6%vs 25.4%±6.2%,21.3%±4.4%vs 35.7%±7.3%,and 7.2%±3.2%vs 19.2%±5.4%,all P<0.01).The PDGF-B positive cell percentage in adventitia peaked 28 days later in Group S and 14 days later in Group NS,and the PDGF-B positive cell percentage in adventitia 28 days later of Group S was significantly higher than that of Group NS(42.5%±8.6%vs 31.9%±4.6%%,P<0.01).RT-PCR analysis revealed that PDGF-B amplification products(457 bp)appeared in both group S and NS from 7 to 28 days,and the PDGF-B mRNA expression levels 7,14,and 28 days later of Group S were all significantly lower than those of Group NS(31.2%±6.5%vs 45.4%±8.4%,P<0.05:42.3%±6.2%vs 65.2%±11.5%,P<0.01:and 21.3%±5.6%vs 36.2%±9.4%,P<0.01).Conclusion Non-restrictive external stenting can inhibit the synthesis of PDGF and change its distribution,which may be one of the mechanisms of external stent in preventing re-stenosis of vein grafts.
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