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Application of molecular method in identification of bacterial endophthalmitis
Author(s): 
Pages: 1429-1432
Year: Issue:  20
Journal: NATIONAL MEDICAL JOURNAL OF CHINA

Keyword:  BacteriumRNASequence;
Abstract: Objective To search for a non-culture method to impmve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases.Methods Fifty clinical specimens of infective endophthalmitis were collected.DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers.The PCR products were sequenced subsequendy.Results Twenty-two(44%)bacterial smears and 13(26%)cultured specimens were positive.PCR positivity was seen in 28 of the 50 cases(56%)and 22 specimens(44%)were sequenced successfully.The results of 16s rRNA gene sequence analysis did not completely conform to the resuhs obtained from culturedependent approach.Conclusion Molecular identification method is a rapid and efficient approach for the diagnosis of bacterial infeetive endophthalmitis.
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