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Determination of Strychnine Content in Strychnos Nuxvomica by the Ultraviolet Spectrophotometric
Author(s): MA mi-xia, LIU Jie-qing, HU Wen-xiang
Pages: 20-
22
Year: 2007
Issue:
9
Journal: CHINA HEALTHCARE INNOVATION
Keyword: 马钱子; 士的宁; 紫外分光光度法; 含量测定;
Abstract: 目的 用紫外分光光度法测定马钱子提取液中士的宁含量,为士的宁含量测定提供了依据.方法 用紫外分光光度计对士的宁标准溶液进行波长扫描,确定最适吸收波长,在此波长下建立士的宁标准品的吸光度-浓度标准曲线.根据标准曲线测定马钱子提取液中士的宁的含量.结果:士的宁最适吸收波长为254nm,吸光度-浓度回归方程为y=36.425x+0.0039,平均加样回收率为99.21%,RSD为2.24%,马钱子提取液中士的宁含量平均值为833mg·g-1.结论 紫外分光光度法测定马钱子提取液中士的宁含量,方法简单、快速,重现性好,可为士的宁质量控制提供依据.
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