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Application of Fluorescein Sodium in Vitreous of Proliferative Diabetic Retinopathy Patients after Taking Fluorescein Sodium Injection Orally
Author(s): 
Pages: 75-77
Year: Issue:  1
Journal: PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY

Keyword:  荧光素钠注射液玻璃体HPLC;
Abstract: 目的 建立增殖性糖尿病视网膜病变患者口服荧光素钠注射液后玻璃体中荧光素钠含量的高效液相色谱测定方法.方法 采用Shim-pack vp-ODS柱(150mm×4.6mm,5μm);流动相:0.1mol·L-1磷酸二氢钠溶液(pH4.4)-甲醇(1:1);流速:1ml/min;激发波长:470nm、发射波长:513nm.结果 人玻璃体中荧光素钠浓度在0.03~0.96μg·ml-1范围内呈良好的线性关系,r=0.997 3.增殖性糖尿病视网膜病变患者口服荧光素钠注射液后玻璃体中荧光素钠含量在0.052~0.375μg·ml-1范围内.结论 方法简便、准确、重现性好,可用于人玻璃体中荧光素钠含量的测定.
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