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A primary study on the biochemical mechanism of degrading keratins by Stenotrophomonas maltophilia DHHJ
Author(s): 
Pages: 70-73,82
Year: Issue:  2
Journal: JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI

Keyword:  角蛋白嗜麦芽窄食单胞菌DHHJ生化机制羽毛水解;
Abstract: 对嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)DHHJ降解角蛋白的生化机制进行初步研究.结果发现:该细菌与羽毛共培养24 h后,电镜下观察到细菌紧密地生长在羽枝上,96 h后羽毛完全降解;该菌所产角蛋白酶属于胞外酶,胞内二硫键还原酶可提高该胞外酶活性3倍左右.单独的角蛋白酶和二硫键还原酶在没有活的细菌存在下,都不能完全降解羽毛,这说明细菌附着在降解过程中起了重要的作用,也可能是由于细菌持续提供了一种还原剂破坏二硫键.此外,羽毛降解过程中,在细菌与羽毛共培养液中检测到亚硫酸盐,说明亚硫酸盐解可能对羽毛降解也起了一定的作用.
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