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Effects of Pollen Typhae Total Flavone on Glucose Metabolism in C2C12 Skeletal Muscle Ceils
Author(s): 
Pages: 39-42
Year: Issue:  2
Journal: ACTA UNIVERSITATIS TRADITIONIS MEDICALIS SINENSIS PHARMACOLOGIAEQUE SHANGHAI

Keyword:  蒲黄总黄酮糖代谢棕榈酸胰岛素抵抗;
Abstract: 目的:探讨蒲黄总黄酮(PTF)对骨骼肌细胞葡萄糖代谢的影响.方法:PTF处理棕榈酸(Palmitate)诱导而致胰岛素抵抗(IR)的C2C12骨骼肌细胞,采用XTT法观察药物对细胞活性的影响;通过检测培养液中的葡萄糖含量反映葡萄糖消耗量;3H-葡萄糖摄入法观察细胞对葡萄糖的转运率.结果:0.25 mmol/Lpalmitate培养16 h,造成C2C12骨骼肌细胞葡萄糖消耗量下降34.66%,0.5g/L PTF可使其葡萄糖消耗量显著增加,且呈一定的时间依赖效应,与罗格列酮(ROS)相比,统计学差异显著(P<0.05);0.25 mmol/L.Palmi-hate培养16 h,使C2C12骨骼肌细胞葡萄糖摄取率下降30.43%,造成骨骼肌细胞胰岛素抵抗(IR)模型,0.5g/L PTF可使IR模型葡萄糖转运率增加32.39%,ROS可使其转运率增加45.88%,与模型组相比,差异有统计学意义(P<0.05).结论:FIT能显著增加C2C12骨骼肌细胞的葡萄糖消耗和摄取,这可能是PTF改善骨骼肌IR的机制之一.
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