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EFFECT OF LOW DOSE PRE-IRRADIATION ON GENETIC MATERICAL DAMAGE CAUSED BY HIGH DOSAGE OF CYCLOPHOSPHAMIDE
Author(s): 
Pages: 44-47
Year: Issue:  1
Journal: ACTA ACADEMIAE MEDICAL QINGDAO UNIVERSITATIS

Keyword:  Radiation doseCyclophosphamideDNA damageMicronuclei;
Abstract: Objective To study the effect of low-dose γ-ray pre-radiation on damages of DNA in peripheral lymphocytes and genetic material due to high-dose cyclophosphamide (CTX). Methods Fifty-five Kunming strain male mice were randomly divided into five groups: blank group, sham-radiation group, low-dose radiation group (LDR group), cyclophosphamide chemotherapy group (CTX group) and LDR+CTX group. Having being raised for one week, all the mice (blank group excluded) were implanted subcutaneously with S180 cells in the left groin. On day 8 and 11, mice in LDR and LDR+CTX groups were given 75 mGy whole-body γ-radiation, 30 hours later, mice in CTX and LDR+CTX groups were given intraperitoneal injection of 3.0 mg cyclophosphamide. All the mice were sacrificed on day 13. DNA damage was analyzed using single cell gel electrophoresis, genetic material damage of bone marrow was analyzed using micronucleus frequency (MNF) of polychromatoerythrocytes (PCE). ResultsCompared with sham-radiation group, the DNA damage in CTX group increased significantly; 75 mGy γ-ray pre-radiation significantly decreased the DNA damage induced by CTX. Compared with control group and sham-irradiation group, MNF of PCE in CTX group increased markedly (t=3.63-5.46,P<0.05); pre-radiation lessened MNF of PCE, but with no statistically significant (P>0.05). Conclusion High-dose CTX may cause damage of DNA in peripheral lymphocytes, which may be prevented by 75 mGy-radiation prior to chemotherapy. CTX has a strong mutagenic action leading to dramatic increase of MNF in bone marrow. 75 mGy γ-ray pre-radiation fails to inhibit the genetic toxicity induced by high-dose CTX chemotherapy.
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