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Rescue of infectious porcine reproductive and respiratory syndrome viruses from full-length cDNA clones with mutated overlapping gene arrangements
Author(s): 
Pages: 81-85
Year: Issue:  2
Journal: CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE

Keyword:  猪繁殖与呼吸综合征病毒重叠序列病毒感染性反向遗传操作;
Abstract: 为了研究重叠序列在病毒繁殖周期中所起的作用以及更好地操作感染性克隆,将猪繁殖与呼吸综合征病毒(PRRSV)感染性克隆ORF间(ORF5/6和ORF6/7)重叠区域分离并插入酶切位点,成功构建两个突变的全长质粒,随后进行了病毒拯救和复制分析.结果表明:(1)两个全长质粒均能够进行病毒拯救,并产生明显细胞病变;(2)突变病毒具遗传稳定性;(3)病毒空斑形态及生长曲线与亲本毒株近似.表明PRRSV ORF间重叠碱基的安排方式对病毒的感染性是非必需的,获得的两株突变病毒为进一步研制PRRSV基因标识疫苗、研究PRRSV各编码蛋白功能奠定了基础.
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