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Expression, purification and identification of the chicken anemia virus vp2
Author(s): 
Pages: 113-117
Year: Issue:  2
Journal: CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE

Keyword:  鸡贫血病毒vp2基因表达纯化;
Abstract: 根据GenBank上已发表的鸡贫血病毒Cux-1株基因组序列设计并合成了1对特异性引物,通过PCR扩增出vp2基因,将扩增出的片段克隆到pGM-T载体上,通过测序分析证实该片段与鸡贫血病毒Cux-1株的vp2基因序列一致.将克隆的vp2基因亚克隆到原核表达载体pET-32a(+)上并转化至大肠杆菌BL21(DE3)中,用IPTG诱导表达,表达的融合蛋白进行SDS-PAGE和Western blot分析,并用镍柱在天然状态下进行了纯化.结果表明,表达的融合蛋白分子量约为45 ku,能与鸡贫血病毒阳性血清发生反应.
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