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Cloning and expression of glycoprotein J gene of infectious laryngotracheitis virus WG strain
Author(s): 
Pages: 118-121
Year: Issue:  2
Journal: CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE

Keyword:  传染性喉气管炎病毒糖蛋白J(gJ)序列分析表达抗原性;
Abstract: 根据GenBank中已发表的传染性喉气管炎病毒全基因组序列(NC 006233)设计引物,以传染性喉气管炎病毒WG株基因组为模板,PCR扩增糖蛋白gJ基因,并对其进行序列分析.用DNAStar软件分析gJ蛋白的抗原性,选择抗原性强的140 aa~483 aa片段,克隆并连接到原核表达载体pET-32a(+)中,转化BL21(DE3)菌株.经IPTG诱导后,获得大小为65.2 ku的重组蛋白,命名为r-gJ,表达量占菌体蛋白总量的60.8%;Western blot分析表明r-gJ具有较强的抗原性.
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