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Development and characterization of monoclonal antibodies against HA protein of H9 influenza virus SD96 strain
Author(s): 
Pages: 149-152,160
Year: Issue:  2
Journal: CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE

Keyword:  禽流感单抗ELISA;
Abstract: 本研究用H9N2亚型禽流感病毒分离株A/Chicken/Shandong/6/1996(CK/SD/6/96)免疫6周龄BALB/c雌性小鼠,取免疫后小鼠脾细胞与骨髓瘤细胞SP2/0融合.采用血凝抑制(HI)和ELISA方法筛选阳性细胞克隆,连续多次克隆培养后获得3株能稳定分泌H9亚型HA特异性单抗的细胞株1F4C4、9F12D1、2C5H12.3株阳性杂交瘤细胞的培养上清及腹水对CK/SD/6/96的HI效价分别为26、24、21和212、210、27.特异性试验表明该3株单抗均不与其他主要禽类病毒和其他14个HA亚型的禽流感病毒发生交叉反应.1F4C4和2C5H12抗体亚类为IgG2b,9F12D1为IgG2a.这3株单抗对H9亚型不同抗原群流感毒株有不同程度的中和作用,表现出单抗识别抗原位点的差异性,为进一步研究H9亚型流感病毒的抗原性差异以及抗原识别提供了物质基础和条件.
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