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Effect of lysophosphatidic acid on differentiation of embryonic neural stem cells into neuroglial cells in rats in vitro
Pages: 759-764
Year: Issue:  6

Keyword:  differentiationneuroglial cellsneural stem cellslysophosphatidic acidlysophosphatidic acid receptors;
Abstract: To study the effect of lysophosphatidic acid(LPA) on the differentiation of embryonic neural stem cells(NSCs)into neuroglial cells in rats in vitro,both oligodendrocytes and astrocytes were detected by their marker proteins galactocerebroside(GalC)and glial fibrillary acidic protein(GFAP),respectively,using double-labeling immunocytochemistry.RT-PCR assay was also used for analyzing the expression of LPA receptors in NSCs.Our results showed that:(1)LPA at different concentrations(0.01-3.0 μmol/L)was added to culture medium and cell counting was carried out on the 7th day in all groups.Exposure to LPA led to a dose-dependent increase of oligodendrocytes with the response peaked at 1.0 μmol/L,with an increased percentage of 32.6%(P<0.01)of total cells as compared to that of 8.5% in the vehicle group.(2)LPA showed no effect on the differentiation of NSCs into astrocytes.(3)RT-PCR assay showed that LPA1 and LPA3 receptors were strongly expressed while LPA2 receptor expressed weakly in NSCs.These results suggest that LPA at low concentration might act as an extracellular signal through the receptors in NSCs,mainly LPA1 and LPA3 receptors,to promote the differentiation of NSCs into oligodendrocytes,while it exhibits little,if any,conceivable effect on the differentiation of NSCs into astrocytes.
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