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Study of apoptosis and expression of proliferating cell nuclear antigen in rabbit liver after high intensity focused ultrasound ablation combined with ultrasound contrast agent
Author(s): LUO Wen, ZHOU Xiao-dong, GONG Xiao-yin, GUO Xiao-hui, ZHANG Jun, QIAN Yun-qiu, HE Jian-guo
Pages: 523-
526
Year: 2007
Issue:
6
Journal: CHINESE JOURNAL OF ULTRASONOGRAPHY
Keyword: 高强度聚焦超声; 造影剂; 细胞凋亡; 增殖细胞核抗原; 肝;
Abstract: 目的 探讨高强度聚焦超声(HIFU)联合超声造影剂消融技术对兔肝组织细胞凋亡和增殖细胞核抗原(PCNA)表达的影响.方法 40只新西兰纯种大白兔分为A、B组, A组兔肝接受HIFU辐照前,经耳缘静脉注射生理盐水;B组接受辐照前注射超声造影剂SonoVue.A、B组均在辐照后5 min、1 d、3 d、7 d、14 d分别处死4只动物,将肝取出体外观察,取靶区及周围组织(距靶区边缘5 mm以内),HE染色观察组织病理学改变,原位末端标记法(TUNEL)检测细胞凋亡,免疫组织化学方法检测细胞PCNA表达.结果 HIFU辐照后3 d、7 d、14 d B组辐照区域边缘纤维包裹带宽度大于A组(P<0.05).HIFU辐照后1 d、3 d、7 d、14 d B组靶区周围组织(距靶区边缘5 mm以内)凋亡细胞数和PCNA阳性细胞指数较A组增高(P<0.05).结论 与单纯HIFU相比,HIFU联合超声造影剂促进了消融后坏死区域机化包裹,增加了HIFU靶区周围细胞凋亡,提高了细胞增殖能力,为进一步增强HIFU消融作用提供了实验数据.
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