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Study on determination of Rape Pollen Polysaccharide content by phenol-sulfuric acid method
Author(s): TU Zongcai, LI Min, LIU Guangxian, DOU Yuxin, ZHANG Bo
Pages: 219-
221
Year: 2007
Issue:
4
Journal: SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY
Keyword: 油菜花粉多糖; 苯酚-硫酸法; 含量测定;
Abstract: 以葡萄糖为对照,优选苯酚-硫酸法测定油菜花粉多糖含量的显色条件,对该法稳定性、精密度、是否受蛋白质干扰等方面进行了研究,得出最佳显色方式为:取待测油菜花粉多糖2mL,加入浓度为5%苯酚1mL,迅速滴加浓硫酸5.0mL,摇匀后于室温放置20min,在482nm处测定吸光度.结果表明,此测定方法简便灵敏,显色稳定,不受蛋白质干扰.
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