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Determination of Salicylic Acid and Chloramphenicol in Compound Chloramphenicol Alcoholic Solution by RP- HPLC
Author(s): 
Pages: 228-230
Year: Issue:  3
Journal: PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY

Keyword:  复方氯霉素醇溶液HPLC水杨酸氯霉素;
Abstract: 目的 建立复方氯霉素醇溶液中水杨酸和氯霉素的含量测定方法.方法 采用反相高效液相色谱法,色谱柱:C18(250mm×4.6mm,5μm);流动相:甲醇-水-冰醋酸(50∶50∶0.1);流速:0.8ml/min;检测波长:295nm;柱温:30℃.结果 水杨酸在0.253 5~2.535 0 μg·ml-1范围内呈良好线性关系,r=0.999 9,平均回收率99.75%,RSD为1.3%;氯霉素在0.255 0~2.550 0 μg·ml-1范围内呈良好线性关系,r=0.999 9,平均回收率99.18%,RSD为1.3%.结论 方法简便、准确,重现性好.
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