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Study on the 1,3-propanediol Fermentation Parameters by Immobilized Cells of the Recombinant Escherichia coli
Author(s): 
Pages: 23-26
Year: Issue:  3
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  13-丙二醇固定化细胞重组大肠杆菌;
Abstract: 利用海藻酸钙将重组大肠杆菌JM109(pHsh-dhaB-dhaG-dhaF-yqhD)固定化,并对影响重组菌固定化细胞发酵的营养因子进行研究.实验结果表明,该重组菌固定化细胞发酵的适宜培养基组成为:甘油80 g/L、酵母膏5.0 g/L、VB120.05 g/L以及KH2PO4 7.5 g/L;在此培养条件下,1,3-丙二醇产量、转化率及生产能力分别可达61.5 g/L、76.8%和2.57 g(L·h),与游离细胞相比,重组菌固定化细胞对底物甘油和产物1,3-丙二醇的耐受力明显提高,且1,3-丙二醇产量明显提高.
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