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Detection of L-Isoleucine in Fermentation Broth by HPLC-ELSD
Author(s): 
Pages: 100-102
Year: Issue:  3
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  L-异亮氨酸高效液相色谱(HPLC)蒸发光散射检测量(ELSD);
Abstract: 采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)定量测定发酵液中L-异亮氨酸的含量.结果表明,L-异亮氨酸浓度在0.960~2.880 g/L时,其峰面积(y)与相应的浓度(X)呈线性关系,线性方程为y=0.661 5X-0.243 9,相关系数为0.999 7.L-异亮氨酸的平均回收率为99.08%~101.3%,相对标准偏差为0.56%~1.32%.方法的精密度、准确度好,稳定性高,能简便、快速、准确地测定发酵液中L-异亮氨酸的含量.
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