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Intermolecular Forces and Secondary Structure of Acid-deamidated Rice Protein
Author(s): Yi Cuiping, Yao Huiyuan
Pages: 1-
4
Year: 2007
Issue:
3
Journal: JOURNAL OF THE CHINESE CEREALS AND OILS ASSOCIATION
Keyword: 大米蛋白; 酸法脱酰胺; 分子间作用力; 二级结构;
Abstract: 大米蛋白经酸法脱酰胺改性引起分子间作用力和二级结构发生变化.对改性蛋白表面疏水性、ζ电位、巯基和二硫键、DSC以及FI-IR图谱分析表明,随着脱酰胺度从0增加到69.4%,大米蛋白的表面疏水性从5 448.5降低到1 433.2,ζ电位从-29.5增大到-52.7.未脱酰胺及低脱酰胺度(6.4%)二硫键含量较高,脱酰胺初期能破坏部分二硫键,当脱酰胺度>19.6%后,脱酰胺对大米蛋白的巯基与二硫键含量不产生影响.随着脱酰胺度增加,蛋白变性的峰值温度降低、热焓增加.此外,酸法脱酰胺对大米蛋白的二级结构有影响,影响结果没有明显变化趋势.
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