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Expression of 4-1BB on T lynphocytes and relationship with TH1/TH2 cytokines from patients with rheumatoid arthritis
Author(s): 
Pages: 285-288
Year: Issue:  3
Journal: CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY

Keyword:  类风湿关节炎T淋巴细胞4-1BBIFN-γIL-4;
Abstract: 目的 探讨体外培养的类风湿关节炎(rheumatoid arthritis,RA)T淋巴细胞上共刺激分子4-1BB的表达,及与其分泌TH1/TH2细胞因子的关系.方法 应用流式细胞术检测体外培养的30例RA患者和20例正常对照者T细胞活化前后4-1BB的表达,并且应用ELISA方法检测培养上清液中IFN-γ、IL-4水平.结果 RA患者CD4+T和CD8+T细胞表达的4-1BB明显高于正常对照组(表达百分率分别为18.56%±4.08%和10.33%±2.13%;1.24%±0.12%和0.87%±0.09%,P<0.01),经抗CD3单抗体外刺激后CD4+T和CD8+T细胞表达的4-1BB均显著高于活化前(表达百分率为33.21%±4.29%和21.35%±8.12%,P<0.01).RA患者CD4+T/CD8+T比值明显升高,而且与4-1BB+CD4+T细胞数呈正相关关系(r=0.84,P<0.01),RA患者T淋巴细胞培养液上清中IFN-γ、IL-4均高于正常对照组(P<0.05),经抗CD3单抗体外刺激后上清液中IFN-γ及IL-4均明显高于活化前,以IFN-γ升高最为显著(P<0.01).而且抗CD3单抗刺激前后4-1BB+CD4+T细胞数与培养上清液中IFN-γ水平均呈正相关关系(r=0.721,r=0.487,P<0.05).结论 类风湿关节炎患者T细胞表达的4-1BB在类风湿关节炎的发生发展中具有重要意义,4-1BB可能通过对CD4+T细胞活化,促使TH1细胞因子分泌,参与关节炎症和免疫损伤.
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