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Clone and Polymorphism Analysis on Part of the Pigs' RBP4 Sequences
Author(s): 
Pages: 11-13
Year: Issue:  2
Journal: ACTA AGRICULTURAE BOREALI-SINICA

Keyword:  RBP4 基因克隆PCR-RFLP;
Abstract: 在猪的RBP4基因的第1外显子和第3外显子处设计引物,成功扩增了该基因的第1内含子和第2内含子,与人和鼠的同源性分别为80%和78%,说明扩增正确.采用PCR-RFLP法对大白猪、杜洛克、长白猪、民猪和杂种猪的RBP4基因进行多态性检测.结果表明:该基因经限制性内切酶Msp Ⅰ酶切后获得3种带型:AA,BB和AB型;除大白猪与民猪、长白猪与杂种猪外,其余各猪种问RBP4基因的基因型频率分布均差异极显著(P<0.01);该基因在长白、大白和杂种猪的Msp Ⅰ位点处于群体不平衡状态,在杜洛克和民猪处于群体平衡状态;基因多态信息含量(PIC)计算,大白、长白、杜洛克、民猪和杂种猪均表现为中度多态性(0.5>PIC>0.25),民猪的B等位基因为优势基因,推测B等位基因与猪的高产性状相关.
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