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Inhibitory effect of antisense oligodeoxynucleotides against hTERT on nude mice transplantation tumor of human tongue Tca8113 cancer cell
Author(s): 
Pages: 91-94
Year: Issue:  2
Journal: JOURNAL OF SOUTHEAST CHINA NATIONAL DEFENCE MEDICAL SCIENCE

Keyword:  端粒酶逆转录酶端粒酶反义寡核苷酸肿瘤细胞培养的;
Abstract: 目的 观察人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)基因反义寡核苷酸对人舌鳞癌Tca8113细胞株裸鼠体内成瘤的影响及其作用机制.方法 实验设反义寡核苷酸组、正义寡核苷酸组、空载体组和空白对照组,后3个组为对照组.采用RT-PCR法检测hTERT基因表达变化,TRAP法测定端粒酶活性,免疫组化法检测细胞增殖核抗原(PCNA)表达量.所有数据用单因素ANOVA方差分析进行统计学检验.结果 hTERT基因反义寡核苷酸组人舌鳞癌Tca8113细胞体内成瘤能力、瘤体体积明显小于对照组,hTERTmRNA和端粒酶活性表达低于对照组(P<0.05).结论 hTERT基因反义寡核苷酸能够抑制人舌鳞癌Tca8113体内成瘤,抑制细胞hTERT基因的转录和端粒酶活化可能是其作用机制之一.
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