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Bone marrow mononuclear cells of adult rat to differentiate into smooth muscle progenitor cells in vitro
Author(s): 
Pages: 128-130,封3
Year: Issue:  2
Journal: CHINESE JOURNAL OF CARDIOVASCULAR REHABILITATION MEDICINE

Keyword:  骨髓细胞干细胞肌细胞平滑肌;
Abstract: 目的:探讨大鼠骨髓单个核细胞在体外经条件培养基诱导定向分化成平滑肌祖细胞的可行性.方法:分离4~6周龄的大鼠胫骨、股骨,以4℃预冷的DMEM培养基冲出骨髓,密度梯度离心法分离骨髓单个核细胞,在血小板源生长因子-BB(PDGF-BB)和碱性成纤维细胞生长因子(b-FGF)作用下,培养8~12d形成贴壁的梭形细胞.采用CD34与α-SMA免疫荧光染色进行鉴定,RT-PCR法测定其α-SMA mRNA表达情况.结果:在PDGF-BB作用下,82%的骨髓单个核细胞α-SMA染色阳性,78%细胞CD34染色阳性,新鲜分离的骨髓单个核细胞不表达α-SMA mRNA,体外培养后表达α-SMA mRNA.结论:体外培养的骨髓单个核细胞能分化为平滑肌祖细胞,可作为研究平滑肌细胞分化和筛选抑制再狭窄药物的工具.
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