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Two steps in situ circulatory perfusion method for isolation of chicken hepatocytes
Author(s): 
Pages: 15-16
Year: Issue:  1
Journal: HEILONGJIANG ANIMAL SCIENCE AND VETERINARY MEDICINE

Keyword:  灌流肝细胞;
Abstract: 试验选用15~18日龄的粤黄鸡苗,采用胶原酶原位二步灌流的方法分离肝细胞.对小鸡破坏大脑处死后,打开腹腔,先用PBS液对小鸡进行门静脉快速灌流,当肝脏呈现苍白略带黄色时改用Ⅳ型胶原酶溶液继续灌流,直至肝包膜与肝组织出现裂隙,即可分离出肝细胞.结果表明:用原位二步灌流法可得到大量活性高且分离较纯的鸡肝细胞.
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