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EXPRESSION OF IL-2 IN COS CELL
Author(s): 
Pages: 192-194
Year: Issue:  1
Journal: JOURNAL OF INNER MONGOLIA AGRICULTURAL UNIVERSITY(NATURAL SCIENCE EDITION)

Keyword:  COS细胞IL-2表达放免分析;
Abstract: 为了进一步证实IL-2的存在,我们对IL-2真核表达载体进行了pC-IL-2双酶切鉴定.采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达.经放射免疫测定了转染细胞上清中IL-2表达量为46.05ng/ml.采用依赖IL-2的CTLL-2细胞作为靶细胞的MTT法测定了IL-2表达产物的活性为86 IU/ml.
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