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Issue:
EXPRESSION OF IL-2 IN COS CELL
Author(s):
ZHOU Tai-ying
,
ZHANG he-ling
,
WANG Xiu-mei
,
BO Xiao-zhen
,
GUO Ying
,
GAO Kai
Pages:
192
-
194
Year:
2007
Issue:
1
Journal:
JOURNAL OF INNER MONGOLIA AGRICULTURAL UNIVERSITY(NATURAL SCIENCE EDITION)
Keyword:
COS细胞
;
IL-2表达
;
放免分析
;
Abstract:
为了进一步证实IL-2的存在,我们对IL-2真核表达载体进行了pC-IL-2双酶切鉴定.采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达.经放射免疫测定了转染细胞上清中IL-2表达量为46.05ng/ml.采用依赖IL-2的CTLL-2细胞作为靶细胞的MTT法测定了IL-2表达产物的活性为86 IU/ml.
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