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ye ti shuang shi ji ce ding xue an zai quan zi dong sheng hua yi shang de yun yong
Author(s): 
Pages: 88-89
Year: Issue:  1
Journal: JOURNAL OF YOUJIANG MEDICAL COLLEGE FOR NATIONALITIES

Keyword:  血氨液体双试剂全自动生化分析仪;
Abstract: 目的 在全自动生化仪上建立液体双试剂连续监测法测定血氨.方法 用连续监测法对pH、谷氨酸脱氢酶浓度等反应条件进行实验研究,并进行方法学评价.结果 双试剂连续监测方法,试剂Ⅰ:含三乙醇胺缓冲液(pH7.4)0.25 mol/L,α-酮戊二酸15 mol/L,NADH 0.3 mmol/L;试剂Ⅱ:含谷氨酸脱氢酶≥160 u/L.线性范围达321 μmol/L,批内Cv=3.4%,批间Cv=8.7%.参考值范围(-x±2SD):10.5~44.1 μmol/L.结论 本方法操作简便,结果准确,方法合理,符合全自动条件,可推广使用.
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