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In Vitro Ovary Culture of Cardiocrinum giganteum
Author(s): 
Pages: 197-200
Year: Issue:  1
Journal: ACTA HORTICULTURAE SINICA

Keyword:  大百合组织培养子房;
Abstract: 以大百合的子房为外植体,研究了小鳞茎诱导及植株再生的方法.结果表明:BA和KT是影响大百合子房分化途径的关键因素,其浓度分别为0.1~1.0 mg/L、2.0~4.0 mg/L和高于4.0 mg/L时,外植体分别分化为愈伤组织、芽和叶.外植体分化的基本培养基以N6、B5为佳.愈伤组织诱导小鳞茎的最佳培养基为MS+0.1~0.5 mg/L NAA +2.5 mg/L BA+2.5 mg/L KT +10%蔗糖+0.7%琼脂.在1/2MS +3%蔗糖+0.7%琼脂+1%活性炭的生根培养基上,生根率为100%.炼苗一周后移栽,长势良好.
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