Author(s): CHENG Jin-hua, WANG Dong, ZHU Hua-bin, HAO Hai-sheng
Pages: 18-20
Year: 2006 Issue:  19
Journal: CHINESE JOURNAL OF ANIMAL SCIENCE
Keyword:  畜牧学; 牛胚胎; 切割取样; 小鼠;
Abstract: 体视显微镜下,采用徒手持金属刀片和自制的玻璃切割针分别对小鼠和牛胚胎进行切割取样,并对取样后的胚胎进行体外培养48 h,其发育率分别为76.7%(46/60)和80%(16/20),两者差异不显著(P＞0.05);奶牛新鲜胚胎切割取样后,胚胎移植妊娠率47.1%(8/17),比对照组妊娠率59.0%(23/39)差异显著(P＜0.05).冷冻-解冻胚胎切割取样后移植妊娠率42.9%(6/17)虽然也比对照组50%低,但是差异不显著(P＞0.05).