Inhibition Effects of IFN -γon TNF-α promoted collagen biosynthesis of OSF-derived fibroblasts
Author(s): WU Ying-fang, PENG Jie-ying, FANG Chang-yun, LIU Shu-fan
Pages: 1672-1675
Year: 2006 Issue: 11
Journal: CHINA JOURNAL OF MODERN MEDICINE
Keyword: IFN-γ; TNF-α; 口腔黏膜下纤维化; 成纤维细胞; 胶原合成; 超微结构;
Abstract: 目的观察γ-干扰素(interferon-γ,IFN-γ)对肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)促正常人颊黏膜成纤维细胞(normal mucous fiblasts,NM-FB)及口腔黏膜下纤维化(oral submucous firosis,OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响,探讨IFN-γ用于治疗OSF的可能性.方法取6例OSF患者及5例正常人颊黏膜,行成纤维细胞原代培养并传代,采用羟脯氨酸试剂盒法观察IFN-γ对TNF-α作用下NM-FB及OSF-FB的胶原合成的影响,并在电镜下观察其超微结构的改变.结果IFN-γ对TNF-α促NM-FB及OSF-FB的胶原合成有明显抑制作用.结论IFN-γ是OSF形成过程中的一种负性调节因子,有可能用于临床治疗OSF.
Pages: 1672-1675
Year: 2006 Issue: 11
Journal: CHINA JOURNAL OF MODERN MEDICINE
Keyword: IFN-γ; TNF-α; 口腔黏膜下纤维化; 成纤维细胞; 胶原合成; 超微结构;
Abstract: 目的观察γ-干扰素(interferon-γ,IFN-γ)对肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)促正常人颊黏膜成纤维细胞(normal mucous fiblasts,NM-FB)及口腔黏膜下纤维化(oral submucous firosis,OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响,探讨IFN-γ用于治疗OSF的可能性.方法取6例OSF患者及5例正常人颊黏膜,行成纤维细胞原代培养并传代,采用羟脯氨酸试剂盒法观察IFN-γ对TNF-α作用下NM-FB及OSF-FB的胶原合成的影响,并在电镜下观察其超微结构的改变.结果IFN-γ对TNF-α促NM-FB及OSF-FB的胶原合成有明显抑制作用.结论IFN-γ是OSF形成过程中的一种负性调节因子,有可能用于临床治疗OSF.
Citations
- Hepatic fibrogenesis VALKOVA M Bratisal Lek Listy
- Idiopathic pulmonary fibrosis:Pathogenesis and therapeutic approaches SELMAN M Durgs
- Factors involved in the regulation of type Ⅰ collagen gene expression:implication in fibrosis GHOSH AK Experimental Biology and Medicine
- TNF-α和IFN-γ对OSF成纤维细胞胶原合成的影响 吴颖芳 中国现代医学杂志
- Effect ofγ-interferon on collagen synthesis by normal and fibrotic human lung fibroblasts NARAYANAN AS Chest
- Potential role of apoptotic macrophages in pulmonary inflammation and fibrosis WANG L Journal of Cellular Physiology
- Expression of stromal-derived facter-1 is decreased by IL-1 and TNF and in dermal wound healing FEDYK ER Journal of Immunology
- 干扰素对膀胱肿瘤细胞膜TNFR表达的影响 王军起 临床泌尿外科杂志
- TNF-α和IFN-γ对OSF成纤维细胞胶原合成的影响 吴颖芳 中国现代医学杂志
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