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Study on the Apparent Activity of L-arabinose Isomerase using Permeabilization of Lactobacillus plantarum cells
Author(s): 
Pages: 17-19
Year: Issue:  10
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  植物乳酸杆菌L-阿拉伯糖异构酶酶活透性化细胞塔格糖;
Abstract: 来源植物乳酸杆菌的L-阿拉伯糖异构酶(L-arabinose isomerase,EC 5.3.1.4.)是一种胞内酶,完整细胞呈现较低的酶活,而经过乙醇、乙醚、甲苯、曲拉通-100和CTAB处理的细胞酶活比完整细胞和超声波破碎的细胞表观酶活力均增加.甲苯透性化细胞优化条件:2%~5%甲苯浓度,15℃保温,作用5 min可得到最大酶活力9.3 U,比超声波破碎的细胞表观酶活力提高5倍.透性化细胞中L-阿拉伯糖异构酶60℃保温2 h,残余酶活80%,比超声波破碎抽提液中的酶对热更为稳定.用透性化细胞作为生物催化剂可有效地转化半乳糖为塔格糖.
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