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Study on Fermentation of VB12 by Propionibacterium shermanii in an In Situ Product Removal Bioreactor
Author(s): 
Pages: 78-81
Year: Issue:  10
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  VB12原位分离无机陶瓷膜活性炭;
Abstract: VB12是谢氏丙酸杆菌的胞内次级代谢产物,其传统发酵方法是分批发酵.发酵液中丙酸的浓度达到10g/L时就会对菌体生长产生抑制作用.因此及时地将丙酸从发酵液中分离出去,将会提高菌体浓度,从而可以显著提高VB12产量.文中将无机陶瓷膜分离技术与谢氏丙酸杆菌发酵耦合,构建了一种新型原位分离发酵系统,通过活性炭吸附成功地将发酵体系中的丙酸分离出去,有效解除了丙酸对菌体生长的抑制作用,实现了VB12的高密度发酵,菌体干重由13.5 g/L提高到35.99 g/L,提高了1.67倍,VB12的产量由33.5 mg/L提高到63.1mg/L,提高了0.88倍.
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