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Comparative Study of Two Methods of DNA Extraction from Different Colour Costal Cartilage in STR Genotyping
Author(s): 
Pages: 436-437
Year: Issue:  6
Journal: JOURNAL OF FORENSIC MEDICINE

Keyword:  Chelex-100法酚-氯仿法DNA提取肋软骨;
Abstract: 目的 比较两种DNA提取法对不同色泽肋软骨的DNA STR分型结果的影响.方法 利用Chelex-100法和酚-氯仿法,分别对30例不同色泽的腐败尸体肋软骨进行DNA提取,STR复合扩增,ABI 3100型基因分析仪对扩增产物进行检测.结果 用酚-氯仿法提取的30例腐败尸体肋软骨,均检测到全部STR基因座的等位基因型.用Chelex-100法提取的肋软骨中,22例(11例白色、8例淡黄色、3例黄色)检测出全部STR基因座的等位基因型;7例(3例黄色、4例黄褐色)检测出部分STR基因座的等位基因型;1例黑灰色的腐败尸体肋软骨,未检测出STR基因座的等位基因型.结论 根据肋软骨的色泽,选择适宜的DNA提取方法.对于颜色较深的肋软骨,用酚-氯仿法进行DNA提取有助于提高其STR基因座的检出率.
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