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Research of Expression Condition of Monellin in the Recombinant E. coli Strain
Author(s): 
Pages: 23-26
Year: Issue:  6
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  大肠杆菌甜蛋白monellin重组菌株表达条件;
Abstract: 将带有monellin基因的重组分泌型表达载体pETMO转入大肠杆菌BL21(DE3),得到重组工程菌株BMC33.经IPTG诱导,其所含有的甜蛋白基因可高效表达.研究不同的表达条件对甜蛋白表达水平的影响,得到优化发酵条件:装液量为50 mL/250 mL三角瓶,当培养液OD值达0.8时,添加诱导剂IPTG至终浓为0.9mmol/L,32℃诱导5 h.此时,甜蛋白monellin表达量可高达细菌可溶性蛋白的44.8%.
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