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Screening and Optimizing of Lactobacillus bulgaricus and Streptococcus thermophilus Wort Enrichment Medium
Author(s): 
Pages: 51-55
Year: Issue:  6
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  嗜热链球菌保加利亚乳杆菌麦芽汁促生长物质增菌培养基优化筛选;
Abstract: 研究了在麦芽汁中添加不同的营养因子对嗜热链球菌S.t-3的细胞生长量的影响,进一步采用正交试验优化筛选出S.t-3的麦芽复合汁增菌培养基,并对保加利亚乳杆菌L.b-DR和L.b-S1进行了验证性增菌试验.结果表明,S.t-3、L.b-DR和L.b-S1在麦芽汁中能进行正常的产酸代谢,大豆蛋白胨、牛肉膏、酵母膏可显著促进S.t-3的细胞生长(P<0.05);K2HPO4不仅可以促进菌体增殖,而且还可缓冲基质pH值的变化,避免了低pH值对乳酸菌的伤害;利用L9(33)正交试验,筛选出S.t-3的麦芽复合汁增菌培养基最佳配比为:在10%的麦芽汁中添加0.5%大豆蛋白胨、0.5%酵母膏、1%牛肉膏、0.2%K2HPO4;在麦芽复合汁增菌培养基中,S.t-3、L.b-DR和L.b-S1,37℃恒温培养16 h,活菌数分别为2.42×109 cfu/mL、2.85×109cfu/mL和4.81×109cfu/mL,与液体MRS培养基的活菌数相当,成本较MRS培养基降低1 000元人民币/t.
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