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INTRODUCTION OF DiI DIOLISTIC ASSAY TO LABEL THE NERVOUS CELL AND GLIA
Author(s): 
Pages: 596-598
Year: Issue:  5
Journal: ACTA ANATOMICA SINICA

Keyword:  神经元神经胶质细胞活体成像DiI散射标记C57/B6J小鼠;
Abstract: 目的 对标记神经元和神经胶质的DiI散射法(DiI diolistic assay)进行改进,使操作更简捷,效果更为理想.方法 用C57/B6J小鼠,对固定或培养的脑片内神经元与神经胶质细胞进行DiI散射法标记.结果 该方法可以显示中枢神经系统内神经元及神经胶质细胞的细微结构,其中包括树突小棘.结论 Dil散射标记法标记细胞的细微结构效果得到改善,可用于对树突棘分析,特别是可以直接在培养的脑片上对神经元与神经胶质进行活体标记.
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