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Establishment and characterization of an embryo cell line of Bombyx mori,BmE-SWU2
Author(s): 
Pages: 719-725
Year: Issue:  5
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  家蚕胚胎细胞系核型生长曲线病毒敏感性;
Abstract: 家蚕反转期胚胎组织经过一年多的原代培养,建立了BmE-SWU2细胞系.该细胞系细胞呈短梭形或圆形,细胞较小,属上皮型贴壁生长细胞系;细胞生长速度快,铺平率高于80%,繁殖力强,细胞群体倍增时间为51.8 h.BmE-SWU2细胞系的二倍体细胞(2n=56)比例达到89.76%,属二倍体细胞系,其染色体具有鳞翅目昆虫染色体的典型特征,中期染色体呈短杆状或颗粒状.BmE-SWU2细胞系对BmNPV高度敏感,TCID50为1.581×10-7.
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