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Cloning and sequencing of cytochrome oxidase Ⅱ gene of Aphis glycines and its application in detecting natural enemies
Author(s): 
Pages: 754-758
Year: Issue:  5
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  大豆蚜细胞色素氧化酶Ⅱ基因克隆天敌捕食性昆虫分子鉴定;
Abstract: 利用通用引物,对大豆蚜Aphis glycines Matsmnura的细胞色素氧化酶Ⅱ(COⅡ)基因序列进行克隆和测序.测得的序列长度为810bp,并与已在GenBank上登录的其他蚜虫COⅡ基因序列进行比较分析,验证其同源性,同时将该序列在GenBank进行了登记(登录号为DQ265743).根据此片段的碱基序列设计了1对大豆蚜特异引物,其扩增片段约为270bp;种特异性检验结果表明,该引物只对大豆蚜具有扩增能力,对其他相关蚜虫种类不具有扩增效果;并用此引物对大豆蚜捕食性天敌进行定性检测,结果表明,在取食过大豆蚜的异色瓢虫Harmonia axyridis、龟纹瓢虫Propylaea japonica、大草蛉Chrysopa septempunctata幼虫以及小花蝽Orius similes成虫和幼虫等捕食性昆虫的中肠中均能检测到大豆蚜的DNA片段;而在未取食蚜虫的上述天敌中却未能扩增出来.
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