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Activity changes of phenoloxidase in haemolymph of Helicoverpa armigera parasitized by Ovomermis sinensis and its purification
Author(s): 
Pages: 878-881
Year: Issue:  5
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  棉铃虫中华卵索线虫酚氧化酶血淋巴纯化;
Abstract: 研究了中华卵索线虫Ovomermis sinensis感染棉铃虫Helicoverpa armigera幼虫后宿主体内酚氧化酶活性的变化.研究结果表明,在感染后的第1天,中华卵索线虫的侵入引起酚氧化酶活性的增加,感染组酶活性是同期对照组的1.12倍;但在随后的寄生期间,中华卵索线虫抑制了宿主的酚氧化酶活性,其中以第5天的抑制最为强烈:同期对照组酶活性是感染组的1.52倍.对酚氧化酶进行了初步的分离纯化,纯化倍数为41.5倍,酶得率为12.7%,比活力为4030.6 U/mg.
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