Author(s): ZHANG Qing-rui, SUN Jian-zhou, REN Ning-hui, DONG Xiao-yu, LIU Zhi-min, ZHAI Min
Pages: 88-90
Year: 2006 Issue:  4
Journal: JOURNAL OF HENAN AGRICULTURAL SCIENCES
Keyword:  芍药; 组织培养; 培养基;
Abstract: 以芍药休眠芽为外植体,进行组织培养试验.结果表明,外植体消毒的最佳方法是用0.1%HgCl2消毒10 min,适宜的启动培养基为1/2MS+GA3 1 mg/L+6-BA 1 mg/L,最佳增殖培养基为1/2MS+6-BA 1 mg/L+KT 0.5 mg/L.采用两步生根法,组培苗生根的适宜培养基为1/2MS+IBA 1 mg/L.