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GYPENOSIEDS INDUCE APOPTOSIS IN HUMAN HEPATOMA CELLS
Author(s): 
Pages: 53-56
Year: Issue:  4
Journal: WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE AND MATERIA MEDICA

Keyword:  绞股蓝总皂甙细胞凋亡Bcl-2Bax;
Abstract: 目的:探讨绞股蓝总皂甙(Gp)对人肝细胞瘤细胞(Huh-7)细胞凋亡的影响及作用机制.方法:采用流式细胞术检测Gp对人Huh-7细胞凋亡的影响,Western blot分析Gp作用Huh-7细胞后表达的Bcl-2,Bcl-XL,Bax和Bad蛋白水平.结果:20mg/ml Gp作用于细胞24h后,64%的Huh-7细胞发生凋亡,而人纤维细胞凋亡率为12%.Western blot结果显示Gp作用后Huh-7细胞中Bcl-2表达明显降低,而Bax表达显著上调.结论:Gp可诱导人Huh-7细胞凋亡,其作用机制是通过下调Bcl-2和上调Bax的表达而发挥作用.
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