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Determination of the Content of Strychnine in Renqingchangjue by RP-HPLC
Author(s): 
Pages: 71-73
Year: Issue:  4
Journal: WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE AND MATERIA MEDICA

Keyword:  仁青常觉士的宁RP-HPLC含量测定;
Abstract: 目的:建立仁青常觉中士的宁的含量测定方法.方法:色谱柱:Diamonsil(TM)钻石C18,(4.6mm×200m,5μm);流动相:甲醇-0.01mol/L磷酸二氢钾溶液(25:75)(用10%磷酸溶液调节pH至2.5);检测波长为254nm;流速:0.8ml/min;柱温:35℃.结果:士的宁色谱峰与其它色谱峰分离良好,进样量在0.03792~0.18960μg范围内,呈良好线性关系(r=0.9999),平均回收率为97.02%,RSD=1.26%(n=6).结论:本法简便快捷,结果可靠,为控制仁青常觉的质量提供一个参考方法.
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