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DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection
Author(s): 
Pages: 196-197,203
Year: Issue:  3
Journal: JOURNAL OF FORENSIC MEDICINE

Keyword:  激光显微捕获(LCM)口腔上皮细胞STR-DNA分型;
Abstract: 目的探索激光显微技术(laser capture microdissection system,LCM)捕获口腔上皮细胞,并进行STR-DNA分型检测的方法.方法用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞,进行Profiler Plus试剂盒STR复合扩增,检测DNA基因型.结果7~8个口腔上皮细胞能成功获得STR-DNA分型.3~4个口腔上皮细胞不能成功获得STR-DNA分型.结论激光显微捕获作为一种分离单个细胞的新技术,对于微量口腔上皮细胞的STR-DNA分型是可行的.
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