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Issue:
Cloning and sequence analysis of ORF2 gene of two PCV-2
Author(s):
ZHANG Jin-chuan
,
PENG Yong-gang
Pages:
4
-
7
Year:
2006
Issue:
3
Journal:
GANSU ANIMAL AND VETERINARY SCIENCES
Keyword:
2型圆环病毒
;
ORF2基因
;
克隆
;
序列分析
;
Abstract:
通过PCR扩增,对两株分离到的2型猪圆环病毒的ORF2基因进行了扩增,将获得的PCR产物分别与T载体连接,获得了两个毒株的ORF2基因阳性克隆.序列分析表明,与其它中国分离株同源性高达99%以上,同处于一个基因簇,但和中国BF分离株同源性却只有92.3%.而荷兰分离的NL_PMWS_2株与中国分离株的同源性却在99%以上,表明中国流行的PCV-2和国外分离株有密切关系.
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