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Protein kinase Cδ is possibly involved in the transition from hypertrophy to apoptosis of myocardiocytes
Author(s): 
Pages: 269-274
Year: Issue:  3
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  心肌细胞肥大细胞凋亡蛋白激酶Cδrottlerin;
Abstract: 为了探讨肥大心肌细胞对凋亡刺激的易感性及蛋白激酶Cδ(protein kinase Cδ,PKCδ)在其中的作用,以内皮素-1(endothelin-1,ET-1)处理原代培养的新生大鼠心肌细胞,诱导心肌细胞肥大;再用血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)作为细胞凋亡诱导因子,采用鬼笔环肽(phalloidin)荧光染色与细胞面积测量两种方法检测心肌肥大,Hoechst 33258荧光染色检测细胞凋亡.结果显示:(1)1与10 nmol/L ET-1作用48 h,心肌细胞肌原纤维排列整齐、染色增浓,随ET-1浓度增加而愈加明显,心肌细胞表面积分别增加42.5%和67.3%,以此作为轻度和中度心肌细胞肥大模型.(2)正常、轻度肥大与中度肥大心肌细胞受1 nmol/L Ang Ⅱ处理24 h后,凋亡率分别为(15.54±1.32)%、(20.65±1.40)%与(29.33±3.52)%,三组之问有显著差异(P<0.05).(3)受Ang Ⅱ刺激后,PKCδ特异性抑制剂rottlerin不影响正常心肌细胞的凋亡率,却有效抑制了轻度和中度肥大心肌细胞的凋亡.肥大心肌细胞凋亡易感性明显高于正常心肌细胞,抑制PKCδ可以抑制肥大心肌细胞凋亡,提示PKCδ参与肥大心肌细胞凋亡过程.
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