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Extraction, purification and immunological activity assay of a polysaccharide from eggs of sea urchin Strongylocentrotus nudus
Author(s): 
Pages: 7-11
Year: Issue:  3
Journal: CHINESE JOURNAL OF MARINE DRUGS

Keyword:  海胆多糖正交实验分离纯化免疫活性;
Abstract: 目的从光棘球海胆中分离纯化海胆黄多糖(polysaccharide from the eggs of Strongylocentrotus nudus,简称SEP),确定其纯度和分子量,并观察其免疫活性.方法海胆黄先经丙酮脱脂,根据正交实验和活性分析确定最佳热水提取条件,然后热水提取、去蛋白、醇沉得海胆黄粗多糖.粗多糖经超滤、DEAE Sepharose Fast Flow及Sephacryl S-400柱层析纯化得多糖精品SEP.经高效液相色谱、聚丙烯酰胺凝胶电泳及纸层析鉴定其纯度.高效凝胶渗透色谱法(HPGPC)测定其分子量.体外脾淋巴细胞增殖实验测定其免疫活性.结果从海胆黄中分离纯化得到的均一多糖组分SEP,经检测其分子量为1950KD左右.脾淋巴细胞增殖实验表明SEP可显著促进脾淋巴细胞的增殖.结论从海胆黄中分离纯化得到的均一多糖组分SEP具有较强的体外免疫活性.
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