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Isolation and characterization of the conotoxins of Conus textile from the South China Sea
Author(s): 
Pages: 22-27
Year: Issue:  3
Journal: CHINESE JOURNAL OF MARINE DRUGS

Keyword:  织锦芋螺芋螺毒素凝胶层析HPLC分离纯化氨基酸序列测定;
Abstract: 目的从中国南海织锦芋螺中分离新的芋螺毒素,为药物发现、药物设计提供新的活性多肽或前体多肽,比较不同地域织锦芋螺毒素的构成,研究环境对织锦芋螺毒素分泌的影响.方法织锦芋螺毒液经预处理后,凝胶层析分离粗毒,用HPLC进一步分离,氨基酸测序.结果分离得到了10余个织锦芋螺毒素组分,确定了10个织锦芋螺毒素的氨基酸序列,其中4个为新型织锦芋螺毒素,6个与已报道的相同.结论中国南海织锦芋螺毒液中含有丰富的M族芋螺毒素,毒素成分与菲律宾宿务岛(Cebu)、马尼拉岛的织锦芋螺相似.
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