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A new method for extracting agarose using basic resin
Author(s): 
Pages: 28-32
Year: Issue:  3
Journal: CHINESE JOURNAL OF MARINE DRUGS

Keyword:  琼胶糖强碱性树脂凝胶强度结晶紫电泳DNA凝胶电泳;
Abstract: 目的研究一种低成本制备琼胶糖的简便方法.方法以江蓠琼胶粉为原料,采用NH4+型阳离子交换树脂脱除1.5%琼胶溶液中的金属离子,再用OH-型强碱性阴离子交换树脂在恒温70℃下吸附沉降琼胶酯,提取出白色琼胶糖.结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标,凝胶强度达到560g/cm2,提取率达57%,测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳.结论本方法提取的琼胶糖具有良好的电泳性能,适合用于生物化学和分子生物学的凝胶电泳研究.本法与EDTA-2Na法和DEAE纤维素法相比,是一种低成本制备琼胶糖的简便方法.
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